Identification of a dominant Chlamydia trachomatis strain in patients attending sexual transmitted infection clinic and female sex workers in Tunisia using a high resolution typing method.
Identifieur interne : 000342 ( Main/Exploration ); précédent : 000341; suivant : 000343Identification of a dominant Chlamydia trachomatis strain in patients attending sexual transmitted infection clinic and female sex workers in Tunisia using a high resolution typing method.
Auteurs : Houda Gharsallah [Tunisie] ; Reinier J M. Bom [Pays-Bas] ; Sylvia M. Bruisten [Pays-Bas] ; Michelle Himschoot [Pays-Bas] ; Olfa Frikha-Gargouri [Tunisie] ; Adnene Hammami [Tunisie]Source :
- Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [ 1567-7257 ] ; 2016.
Descripteurs français
- KwdFr :
- Adulte (MeSH), Analyse de regroupements (MeSH), Analyse de séquence d'ADN (MeSH), Chlamydia trachomatis (classification), Chlamydia trachomatis (génétique), Femelle (MeSH), Génotype (MeSH), Humains (MeSH), Hétérosexualité (MeSH), Infections à Chlamydia (microbiologie), Infections à Chlamydia (épidémiologie), Jeune adulte (MeSH), Mâle (MeSH), Pays-Bas (épidémiologie), Travailleurs du sexe (MeSH), Tunisie (épidémiologie), Typage par séquençage multilocus (MeSH), Variation génétique (MeSH).
- MESH :
- génétique : Chlamydia trachomatis.
- microbiologie : Infections à Chlamydia.
- épidémiologie : Infections à Chlamydia, Pays-Bas, Tunisie.
- classification : Adulte, Analyse de regroupements, Analyse de séquence d'ADN, Chlamydia trachomatis, Femelle, Génotype, Humains, Hétérosexualité, Jeune adulte, Mâle, Travailleurs du sexe, Typage par séquençage multilocus, Variation génétique.
English descriptors
- KwdEn :
- Adult (MeSH), Chlamydia Infections (epidemiology), Chlamydia Infections (microbiology), Chlamydia trachomatis (classification), Chlamydia trachomatis (genetics), Cluster Analysis (MeSH), Female (MeSH), Genetic Variation (MeSH), Genotype (MeSH), Heterosexuality (MeSH), Humans (MeSH), Male (MeSH), Multilocus Sequence Typing (MeSH), Netherlands (epidemiology), Sequence Analysis, DNA (MeSH), Sex Workers (MeSH), Tunisia (epidemiology), Young Adult (MeSH).
- MESH :
- classification : Chlamydia trachomatis.
- epidemiology : Chlamydia Infections, Netherlands, Tunisia.
- genetics : Chlamydia trachomatis.
- microbiology : Chlamydia Infections.
- Adult, Cluster Analysis, Female, Genetic Variation, Genotype, Heterosexuality, Humans, Male, Multilocus Sequence Typing, Sequence Analysis, DNA, Sex Workers, Young Adult.
Abstract
BACKGROUND
The distribution of Chlamydia trachomatis genotypes in Tunisia was previously studied using the reverse hybridization method. In this study, we used multilocus sequence typing (MLST) to describe Chlamydia trachomatis genetic diversity among heterosexual populations in Tunisia. The obtained sequence types (STs) were compared with those from a heterosexual population from Amsterdam, the Netherlands.
METHODS
Clinical Tunisian patients and female sex workers provided 107 Chlamydia trachomatis positive samples that were used for MLST. Samples from 256 heterosexuals visiting the Amsterdam STI clinic were included as a reference group. Six highly variable genetic regions including the ompA gene were amplified and sequenced. The ST numbers were derived from a Chlamydia typing database (http://mlstdb.uu.se) and used to draw minimum spanning trees.
RESULTS
ompA sequencing detected 7 genotypes among the Tunisian populations of which genotype E was the most prevalent (66.3%). This genotype E resolved into 23 different STs and among these the ST3 was predominant (53.5%). MLST displayed 43 STs, of which 28 (65%) were new in the database. Minimum spanning tree analysis of all Tunisian samples identified 4 clusters of which one formed a clonal cluster with samples presenting the most prevalent ST3. When comparing samples from the Tunisian and Dutch populations in one minimum spanning tree, there was little overlap between the Chlamydia trachomatis samples.
CONCLUSION
The CT-hrMLST scheme allowed us to identify that the Tunisian distribution was dominated by one genotype E (ST3) strain which is also highly prevalent in many other countries worldwide.
DOI: 10.1016/j.meegid.2016.08.002
PubMed: 27497657
Affiliations:
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Electronic address: houda_gharsallah@yahoo.fr.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Department of Microbiology and research laboratory "Microorganismes et Pathologies Humaines", Habib Bourguiba University Hospital, Medical School of Sfax, Avenue Majida Boulila 3000, University of Sfax</wicri:regionArea><wicri:noRegion>University of Sfax</wicri:noRegion></affiliation></author><author><name sortKey="Bom, Reinier J M" sort="Bom, Reinier J M" uniqKey="Bom R" first="Reinier J M" last="Bom">Reinier J M. Bom</name><affiliation wicri:level="1"><nlm:affiliation>Public Health Laboratory, Public Health Service of Amsterdam (GGD Amsterdam), Nieuwe Achtergracht 100, 1018 WT Amsterdam, The Netherlands. Electronic address: reinierbom@gmail.com.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Public Health Laboratory, Public Health Service of Amsterdam (GGD Amsterdam), Nieuwe Achtergracht 100, 1018 WT Amsterdam</wicri:regionArea><wicri:noRegion>1018 WT Amsterdam</wicri:noRegion></affiliation></author><author><name sortKey="Bruisten, Sylvia M" sort="Bruisten, Sylvia M" uniqKey="Bruisten S" first="Sylvia M" last="Bruisten">Sylvia M. Bruisten</name><affiliation wicri:level="1"><nlm:affiliation>Public Health Laboratory, Public Health Service of Amsterdam (GGD Amsterdam), Nieuwe Achtergracht 100, 1018 WT Amsterdam, The Netherlands. 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Electronic address: mhimschoot@ggd.amsterdam.nl.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Public Health Laboratory, Public Health Service of Amsterdam (GGD Amsterdam), Nieuwe Achtergracht 100, 1018 WT Amsterdam</wicri:regionArea><wicri:noRegion>1018 WT Amsterdam</wicri:noRegion></affiliation></author><author><name sortKey="Frikha Gargouri, Olfa" sort="Frikha Gargouri, Olfa" uniqKey="Frikha Gargouri O" first="Olfa" last="Frikha-Gargouri">Olfa Frikha-Gargouri</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and research laboratory "Microorganismes et Pathologies Humaines", Habib Bourguiba University Hospital, Medical School of Sfax, Avenue Majida Boulila 3000, University of Sfax, Tunisia; Biopesticides Team, Centre of Biotechnology of Sfax, Road of Sidi Mansour Km 6, 3018 Sfax, Tunisia. Electronic address: olfafrikhagagouri@ymail.com.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Department of Microbiology and research laboratory "Microorganismes et Pathologies Humaines", Habib Bourguiba University Hospital, Medical School of Sfax, Avenue Majida Boulila 3000, University of Sfax, Tunisia; Biopesticides Team, Centre of Biotechnology of Sfax, Road of Sidi Mansour Km 6, 3018 Sfax</wicri:regionArea><wicri:noRegion>3018 Sfax</wicri:noRegion></affiliation></author><author><name sortKey="Hammami, Adnene" sort="Hammami, Adnene" uniqKey="Hammami A" first="Adnene" last="Hammami">Adnene Hammami</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and research laboratory "Microorganismes et Pathologies Humaines", Habib Bourguiba University Hospital, Medical School of Sfax, Avenue Majida Boulila 3000, University of Sfax, Tunisia. Electronic address: adnene.hammami@rns.tn.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Department of Microbiology and research laboratory "Microorganismes et Pathologies Humaines", Habib Bourguiba University Hospital, Medical School of Sfax, Avenue Majida Boulila 3000, University of Sfax</wicri:regionArea><wicri:noRegion>University of Sfax</wicri:noRegion></affiliation></author></analytic><series><title level="j">Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases</title><idno type="eISSN">1567-7257</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult (MeSH)</term><term>Chlamydia Infections (epidemiology)</term><term>Chlamydia Infections (microbiology)</term><term>Chlamydia trachomatis (classification)</term><term>Chlamydia trachomatis (genetics)</term><term>Cluster Analysis (MeSH)</term><term>Female (MeSH)</term><term>Genetic Variation (MeSH)</term><term>Genotype (MeSH)</term><term>Heterosexuality (MeSH)</term><term>Humans (MeSH)</term><term>Male (MeSH)</term><term>Multilocus Sequence Typing (MeSH)</term><term>Netherlands (epidemiology)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Sex Workers (MeSH)</term><term>Tunisia (epidemiology)</term><term>Young Adult (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte (MeSH)</term><term>Analyse de regroupements (MeSH)</term><term>Analyse de séquence d'ADN (MeSH)</term><term>Chlamydia trachomatis (classification)</term><term>Chlamydia trachomatis (génétique)</term><term>Femelle (MeSH)</term><term>Génotype (MeSH)</term><term>Humains (MeSH)</term><term>Hétérosexualité (MeSH)</term><term>Infections à Chlamydia (microbiologie)</term><term>Infections à Chlamydia (épidémiologie)</term><term>Jeune adulte (MeSH)</term><term>Mâle (MeSH)</term><term>Pays-Bas (épidémiologie)</term><term>Travailleurs du sexe (MeSH)</term><term>Tunisie (épidémiologie)</term><term>Typage par séquençage multilocus (MeSH)</term><term>Variation génétique (MeSH)</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Chlamydia trachomatis</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Chlamydia Infections</term><term>Netherlands</term><term>Tunisia</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Chlamydia trachomatis</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Chlamydia trachomatis</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Infections à Chlamydia</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Chlamydia Infections</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Infections à Chlamydia</term><term>Pays-Bas</term><term>Tunisie</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Cluster Analysis</term><term>Female</term><term>Genetic Variation</term><term>Genotype</term><term>Heterosexuality</term><term>Humans</term><term>Male</term><term>Multilocus Sequence Typing</term><term>Sequence Analysis, DNA</term><term>Sex Workers</term><term>Young Adult</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="fr"><term>Adulte</term><term>Analyse de regroupements</term><term>Analyse de séquence d'ADN</term><term>Chlamydia trachomatis</term><term>Femelle</term><term>Génotype</term><term>Humains</term><term>Hétérosexualité</term><term>Jeune adulte</term><term>Mâle</term><term>Travailleurs du sexe</term><term>Typage par séquençage multilocus</term><term>Variation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>The distribution of Chlamydia trachomatis genotypes in Tunisia was previously studied using the reverse hybridization method. In this study, we used multilocus sequence typing (MLST) to describe Chlamydia trachomatis genetic diversity among heterosexual populations in Tunisia. The obtained sequence types (STs) were compared with those from a heterosexual population from Amsterdam, the Netherlands.</p></div><div type="abstract" xml:lang="en"><p><b>METHODS</b></p><p>Clinical Tunisian patients and female sex workers provided 107 Chlamydia trachomatis positive samples that were used for MLST. Samples from 256 heterosexuals visiting the Amsterdam STI clinic were included as a reference group. Six highly variable genetic regions including the ompA gene were amplified and sequenced. The ST numbers were derived from a Chlamydia typing database (http://mlstdb.uu.se) and used to draw minimum spanning trees.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>ompA sequencing detected 7 genotypes among the Tunisian populations of which genotype E was the most prevalent (66.3%). This genotype E resolved into 23 different STs and among these the ST3 was predominant (53.5%). MLST displayed 43 STs, of which 28 (65%) were new in the database. Minimum spanning tree analysis of all Tunisian samples identified 4 clusters of which one formed a clonal cluster with samples presenting the most prevalent ST3. When comparing samples from the Tunisian and Dutch populations in one minimum spanning tree, there was little overlap between the Chlamydia trachomatis samples.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>The CT-hrMLST scheme allowed us to identify that the Tunisian distribution was dominated by one genotype E (ST3) strain which is also highly prevalent in many other countries worldwide.</p></div></front></TEI><affiliations><list><country><li>Pays-Bas</li><li>Tunisie</li></country></list><tree><country name="Tunisie"><noRegion><name sortKey="Gharsallah, Houda" sort="Gharsallah, Houda" uniqKey="Gharsallah H" first="Houda" last="Gharsallah">Houda Gharsallah</name></noRegion><name sortKey="Frikha Gargouri, Olfa" sort="Frikha Gargouri, Olfa" uniqKey="Frikha Gargouri O" first="Olfa" last="Frikha-Gargouri">Olfa Frikha-Gargouri</name><name sortKey="Hammami, Adnene" sort="Hammami, Adnene" uniqKey="Hammami A" first="Adnene" last="Hammami">Adnene Hammami</name></country><country name="Pays-Bas"><noRegion><name sortKey="Bom, Reinier J M" sort="Bom, Reinier J M" uniqKey="Bom R" first="Reinier J M" last="Bom">Reinier J M. Bom</name></noRegion><name sortKey="Bruisten, Sylvia M" sort="Bruisten, Sylvia M" uniqKey="Bruisten S" first="Sylvia M" last="Bruisten">Sylvia M. Bruisten</name><name sortKey="Himschoot, Michelle" sort="Himschoot, Michelle" uniqKey="Himschoot M" first="Michelle" last="Himschoot">Michelle Himschoot</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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